Selection of a suitable explant material for callus induction and in vitro morphogenesis in chir pine (Pinus roxburghii)
Keywords:
Adventitious buds, bud-forming capacity, callus induction, chir pine, DCR mediumAbstract
The present study aimed at standardizing in vitro conditions for callus induction, maintenance and plant regeneration in Pinus roxburghii Sargent. Various factors, including the type of explant, callus induction medium, and explant collection time, were investigated in callus induction. Shoot apical domes were responsive for callus induction with the highest induction frequencies (82.5%) than secondary needles (75.80%) or other tissues on DCR medium supplemented with 22.62 µM 2, 4-Dichlorophenoxyacetic acid (2, 4-D), 26.85 µM α-Naphthalene acetic acid (NAA), and 2 µM Thidiazuron (TDZ) after 18 days. The maximum rate of callus induction was observed in January (for apical domes) and March (for secondary needles), indicating that these collection times are suitable for callus induction from vegetative tissues. Off-white and friable calluses with excellent proliferation were achieved with TDZ better than 6-Benzylaminopurine (BAP) for callus induction and maintenance.
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Copyright (c) 2025 Sadia Kaleem, Aroosha Ashraf, Faheem Aftab (Author)
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